Term of Gsdf. (A) Real-go out PCR away from Gsdf from inside the XX and XY fry from 2 weeks ahead of hatching (dbh) so you’re able to ten weeks shortly after hatching (dah). Blue bars, expression of PG5 to your Y chromosome (Gsdf Y ); purple, the fresh new X chromosome (Gsdf X ). High variations was reviewed by using several-method analysis regarding difference (ANOVA) with Bonferroni post-tests. Columns and you can error taverns show suggest ± SEM (n = cuatro for each developmental stage). (b–d) For the situ hybridization from Gsdf from the gonad. (B) Term out-of Gsdf from inside the 5-dah XY fry. Gsdf are understood merely during the somatic muscle encompassing germ structure. (C) Expression away from Gsdf inside the 5-dah XX fry. Weakened signals had been detected. (D) Gsdf expression for the adult testis. Strong indicators have been sensed in Sertoli muscle (arrowheads) encompassing spermatogonia. Smaller signals had been receive within efferent duct (ed). (E) Gsdf phrase for the mature ovary. Read more